| Proje Ekibi (2) |
Ayşe Elif BÜYÜKBAYRAM
Karabük Üniversitesi, Türkiye Projedeki Rolü: Yürütücü |
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Saseeyah Mıftah Abdulsalam ALREEBAA
Karabük Üniversitesi, Türkiye Projedeki Rolü: Araştırmacı |
| Proje Türü | Yükseköğretim Kurumları Tarafından Destekli Bilimsel Araştırma Projesi |
| Proje No | FYL-2020-2050 |
| Proje Konusu | PEDOT and hydroxymethyl-PEDOT polymers were coated over a platinum electrode and new enzyme electrodes were produced that are not found in literature. The formation of polymerization was shown by cyclic voltammetry. Kinetic parameters of immobilized polyphenol oxidase enzyme were determined. The effect of reaction conditions over enzyme activity were examined. Operational stability and shelf-life of electrodes were also determined. Calibration graph was plotted. LOD values and the working intervals were obtained. In total phenolic material analysis, the results found for peach extracts have been confirmed by Folin-Ciocalteau method. Comparison the performances of two electrodes points that Pt/MeOH-PEDOT/PPO is superior to Pt/PEDOT/PPO with respect to kinetic parameters and limit of detection. Vmax, maximum reaction rate of Pt/MeOH-PEDOT/PPO electrode has a value double of Pt/PEDOT/PPO electrode, which indicates that in MeOH-PEDOT environment, enzyme can increase the reaction rate more easily than PEDOT matrix. Moreover, Km is much smaller in Pt/MeOH-PEDOT/PPO electrode, which means that affinity between enzyme and substrate is much stronger in MeOH-PEDOT matrix. Furthermore, detection limit obtained from Pt/MeOH-PEDOT/PPO electrode is lower than Pt/PEDOT/PPO. However, optimizations exhibit that Pt/PEDOT/PPO electrode works better in milder pH's and temperatures. |
| Proje Durumu | Tamamlandı |
| Başlangıç Tarihi | 18-03-2020 |
| Bitiş Tarihi | 07-04-2021 |
| Anahtar Kelimeler | iletken polimer, polifenol oksidaz, enzim elektrodu, biyosensör |
