| Yazarlar (6) |
Prof. Dr. Müslüm KUZU
Ağrı İbrahim Çeçen Üniversitesi, Türkiye |
Abdulselam Aslan
Giresun Üniversitesi, Türkiye |
|
Ishtıaq Ahmed
Türkiye |
|
Veysal Çomaklı
Ağrı İbrahim Çeçen Üniversitesi, Türkiye |
|
Ramazan Demirdağ
Ağrı İbrahim Çeçen Üniversitesi, Türkiye |
|
Naim Uzun
Ağrı İbrahim Çeçen Üniversitesi, Türkiye |
| Özet |
| Glucose-6-phosphate dehydrogenase (G6PD) and glutathione reductase (GR) are metabolically quite important enzymes. Within this study, these two enzymes were purified for the first time from the gills of Lake Van fish. In the purifying process, ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity column chromatography techniques for glucose-6-phosphate dehydrogenase, temperature degradation and 2',5'-ADP Sepharose 4B affinity column chromatography for glutathione reductase enzyme were used. The control of the enzyme purity and determination of molecular weight were done with sodium dodecyl sulfate polyacrylamide gel electrophoresis. K(M) and V(max) values were determined with Lineweaver-Burk plot. Besides, the effects of some chalcone derivatives on the purified enzymes were analyzed. For the ones showing inhibition effect, % activity-[I] figures were drawn and IC50 values were determined. K(i) value was calculated by using Cheng-Prusoff equation. |
| Anahtar Kelimeler |
| Chalcone | Glucose-6-phosphate dehydrogenase | Glutathione reductase | Inhibition | Lake Van fish |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | FISH PHYSIOLOGY AND BIOCHEMISTRY |
| Dergi ISSN | 0920-1742 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2016 |
| Cilt No | 42 |
| Sayı | 2 |
| Sayfalar | 483 / 491 |
| Doi Numarası | 10.1007/s10695-015-0153-7 |
| Makale Linki | http://link.springer.com/10.1007/s10695-015-0153-7 |
| Atıf Sayıları | |
| WoS | 7 |
| Google Scholar | 13 |
