| Yazarlar (14) |
Murat Çankaya
Erzincan Üniversitesi, Türkiye |
|
Ama Martinez Hernandez
Türkiye |
|
Mehmet Çiftci
Bingöl Üniversitesi, Türkiye |
|
Şükrü Beydemir
Atatürk Üniversitesi, Türkiye |
|
Hasan Özdemir
Atatürk Üniversitesi, Türkiye |
|
Harun Budak
Atatürk Üniversitesi, Türkiye |
|
İlhami Gülçin
Atatürk Üniversitesi, Türkiye |
|
Veysal Çomaklı
Ağrı İbrahim Çeçen Üniversitesi, Türkiye |
|
Tufan Emircupani
Türkiye |
|
Deniz Ekinci
Ondokuz Mayıs Üniversitesi, Türkiye |
Prof. Dr. Müslüm KUZU
Ağrı İbrahim Çeçen Üniversitesi, Türkiye |
|
Qiuhong Jiang
Türkiye |
|
Gregor Eichele
Türkiye |
|
Ömer İrfan Küfrevioğlu
Atatürk Üniversitesi, Türkiye |
| Özet |
| In situ hybridization (ISH) is a powerful method for visualizing gene expression patterns at the organismal level with cellular resolution. When automated, it is capable of determining the expression of a large number of genes. The expression patterns of 662 genes that encode enzymes were determined by ISH in the mid-gestation mouse embryo, a stage that models the complexity of the adult organism. Forty-five percent of transcripts encoding metabolic enzymes (n = 297) showed a regional expression pattern. A similar percentage was found for the 190 kinases that were also analyzed. Many mRNAs encoding glycolytic and TCA cycle enzymes exhibited a characteristic expression pattern. The annotated expression patterns were deposited on the Genepaint database and are retrievable by user-defined queries including gene name and sites of expression. The 662 expression patterns discussed here comprised gene products with activities associated with catalysis. Preliminary analysis of these data revealed that a significant number of genes encoding housekeeping functions such as biosynthesis and catabolism were expressed regionally, so they could be used as tissue-specific gene markers. We found no difference in tissue specificity between mRNAs encoding housekeeping functions and those encoding components of signal transduction pathways, as exemplified by the kinases. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | BMC GENOMICS |
| Dergi ISSN | 1471-2164 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2007 |
| Cilt No | 8 |
| Sayı | 1 |
| Sayfalar | 232 / 246 |
| Doi Numarası | 10.1186/1471-2164-8-232 |
| Makale Linki | http://www.biomedcentral.com/1471-2164/8/232 |
