| Yazarlar (6) |
Dr. Öğr. Üyesi Ümit YILMAZ
Karabük Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Nesibe YILMAZ
Karabük Üniversitesi, Türkiye |
Kevser Tanbek
İnönü Üniversitesi, Türkiye |
|
Hayriye Arzu Ergen
İstanbul Üniversitesi, Türkiye |
|
Nihat Aksakal
İstanbul Üniversitesi, Türkiye |
|
Şakir Ümit Zeybek
İstanbul Üniversitesi, Türkiye |
| Özet |
| This study was aimed to investigate the relationship of miR-17-5p, miR-30b, miR-30d, miR-216a and miR-216b associated with autophagy gene beclin 1, and beclin 1 gene with colorectal cancer (CRC). Forty-seven patients with CRC and 50 healthy individuals with no cancer history were included in this study. In the serum, tumor and non-tumoral tissue samples of the CRC patients, and in the serum samples of the healthy subjects, expression levels of miRNAs were detected by qRT-PCR. The beclin 1 gene expression levels were determined by qRT-PCR, and protein levels were determined by Western blot method in tumor and non-tumor tissue samples of the patients. The miR-17-5p and miR-30d expressions were found to be higher in tumor tissue as compared to patient non-tumor tissues, while expressions of beclin-1, miR-30b and miR-216a were found to be lower. In addition, the beclin-1 protein levels were significantly decreased in the tumor tissue as compared to those in the patient non-tumor tissues. The miR-30d expression was significantly reduced in the serum of the patients when the serum samples of CRC patients and healthy controls were compared. The beclin 1 gene may play a role as a tumor suppressor in CRC. Moreover, these miRNAs cannot be used as highly reliable biomarkers in serum for CRC diagnosis (Tab. 2, Fig. 6, Ref. 46). |
| Anahtar Kelimeler |
| colorectal cancer | autophagy | beclin-1 | miRNA | biomarker |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Bratislava Medical Journal |
| Dergi ISSN | 1336-0345 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 08-2022 |
| Cilt No | 123 |
| Sayı | 9 |
| Sayfalar | 634 / 640 |
| Doi Numarası | 10.4149/BLL_2022_102 |
| Makale Linki | http://dx.doi.org/10.4149/bll_2022_102 |
| Atıf Sayıları | |
| WoS | 1 |
