Characterization of Monoclonal Antibody N-Glycan Conformations by Novel Hydrophilic Interaction Liquid Chromatography (HILIC) with Trapped Ion Mobility Mass Spectrometry (TIMS) and Fluorescence Detection (FLD)
Yazarlar (4)
Izzet Avci
Hacettepe Üniversitesi, Türkiye
Hacettepe Üniversitesi, Türkiye
Mehmet Atakay Hacettepe Üniversitesi, Türkiye
Doç. Dr. Hacı Mehmet KAYILI Karabük Üniversitesi, Türkiye
Bekir Salih Hacettepe Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Analytical Letters (Q3) | ||
| Dergi ISSN | 0003-2719 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 07-2025 |
| Cilt / Sayı / Sayfa | 58 / 12 / 2158–2173 | DOI | 10.1080/00032719.2024.2384700 |
| Makale Linki | https://doi.org/10.1080/00032719.2024.2384700 | ||
| UAK Araştırma Alanları |
Analitik Kimya
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| Özet |
| Monoclonal antibodies (mAbs) have become prevalent in the pharmaceutical sector for treating various diseases and have a significant market presence. The determination of these molecules is complex and challenging. It is necessary to employ high-throughput technologies to characterize these pharmaceuticals in order to characterize sequencing, structure, composition, conformation, and mass. It is important to perform an N-glycosylation study on these macromolecules as their N-glycan profiles have a significant impact on their effectiveness. However, the conformational features of the N-glycans on mAb are not adequately addressed in commonly used methods. This study incorporated ion-mobility mass spectrometry as an additional dimension to established hydrophilic liquid chromatography trapped ion mobility spectrometry with fluorescence detection ((HILIC)LC/TIMS/FLD) in order to enhance the … |
| Anahtar Kelimeler |
| High-performance liquid chromatography (HPLC) | hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD) | monoclonal antibodies | N-glycan | trapped ion-mobility mass spectrometry (TIMS) |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 2 |
| Scopus | 2 |
| Google Scholar | 3 |