Poly(amidoamine) dendrimer‐coated magnetic nanoparticles for the fast purification and selective enrichment of glycopeptides and glycans
Yazarlar (4)
Doç. Dr. Hacı Mehmet KAYILI Karabük Üniversitesi, Türkiye
Prof. Dr. Ali Serol Ertürk Adıyaman Üniversitesi, Türkiye
Gökhan Elmacı Adıyaman Üniversitesi, Türkiye
Prof. Dr. Bekir Salih Hacettepe Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Separation Science (Q2) | ||
| Dergi ISSN | 1615-9306 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2019 |
| Kabul Tarihi | 01-08-2019 | Yayınlanma Tarihi | 27-08-2019 |
| Cilt / Sayı / Sayfa | 42 / 20 / 3209–3216 | DOI | 10.1002/jssc.201900492 |
| Makale Linki | https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201900492 | ||
| UAK Araştırma Alanları |
Analitik Kimya
|
||
| Özet |
| Glycosylated proteins modulate various important functions of organisms. To reveal the functions of glycoproteins, in‐depth characterization studies are necessary. Although mass spectrometry is a very efficient tool for glycoproteomic and glycomic studies, efficient sample preparation methods are required prior to analyses. In the study, poly(amidoamine) dendrimer‐coated magnetic nanoparticles were presented for the specific enrichment and fast purification of glycopeptides and glycans. The enrichment and purification performance of the developed method was evaluated both at the glycopeptide, and the glycan level using several standard glycoprotein digests and released glycan samples. The poly(amidoamine) dendrimer‐coated magnetic nanoparticles not only showed selective affinity (Immunoglobulin G/Bovine Serum Albumin, 1/10 by weight) to glycopeptides and released glycans but also good sensitivity … |
| Anahtar Kelimeler |
| glycan purification | glycopeptide enrichment | glycosylation | mass spectrometry | poly(amidoamine) dendrimer |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 23 |
| Scopus | 28 |
| Google Scholar | 34 |