Hydrophilic spacer-arm containing magnetic nanoparticles for immobilization of proteinase K: Employment for speciation of proteins for mass spectrometry-based analysis
Yazarlar (5)
Prof. Dr. Gulay Bayramoglu Gazi Üniversitesi, Türkiye
Doç. Dr. Hacı Mehmet KAYILI Karabük Üniversitesi, Türkiye
Öğr. Gör. Merve Oztekin Gazi Üniversitesi, Türkiye
Prof. Dr. Bekir Salih Hacettepe Üniversitesi, Türkiye
M. Yakup Arica
Gazi Üniversitesi, Türkiye
Gazi Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Talanta (Q1) | ||
| Dergi ISSN | 0039-9140 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2020 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-01-2020 |
| Cilt / Sayı / Sayfa | 206 / 1 / 120218–0 | DOI | 10.1016/j.talanta.2019.120218 |
| Makale Linki | http://dx.doi.org/10.1016/j.talanta.2019.120218 | ||
| UAK Araştırma Alanları |
Analitik Kimya
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| Özet |
| Proteinase K (ProK) is used for the degradation of proteins in cell lysates to isolate nucleic acids, and for the speciation of proteins for mass spectrometry analysis. In this work, a novel and sensitive immobilization process was developed for examination of protein mixtures by combining MALDI-ToF-MS and nLC-TIMS-ToF-MS/MS systems. To achieve these goals, magnetic nanoparticles (MPs) were prepared via thermal coprecipitation reaction under alkaline condition. The MPs were grafted with a silica layer (i.e., 3-(2,3-epoxypropoxy) propyltrimethoxysilane; EPTES) containing reactive epoxy groups. Then, the silica-grafted magnetic particles were coated with a long chain hydrophilic poly(ethylene glycol) diamine polymer (PEGDAP). The prepared materials were characterized by the Brunauer–Emmett–Teller (BET) method, X-ray diffraction (XRD), scanning electron microscopy (SEM) and attenuated total reflection … |
| Anahtar Kelimeler |
| Immobilized proteinase K | Magnetic nanoparticles | MALDI-ToF-MS | Peptide synthesis | Protein speciation |
Science Direct
Hydrophilic spacer-arm containing magnetic nanoparticles for immobilization of proteinase K: Employment for speciation of proteins for mass spectrometry-based analysis
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 13 |
| Scopus | 14 |
| Google Scholar | 16 |