A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS
Yazarlar (3)
Doç. Dr. Hacı Mehmet KAYILI Karabük Üniversitesi, Türkiye
A. V.C.I. İzzet
Hacettepe Üniversitesi, Türkiye
Hacettepe Üniversitesi, Türkiye
Salih Bekir
Hacettepe Üniversitesi, Türkiye
Hacettepe Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Pharmaceutical and Biomedical Analysis (Q2) | ||
| Dergi ISSN | 0731-7085 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2019 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-09-2019 |
| Cilt / Sayı / Sayfa | 174 / 1 / 191–197 | DOI | 10.1016/j.jpba.2019.05.061 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0731708519311999 | ||
| UAK Araştırma Alanları |
Analitik Kimya
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| Özet |
| Enrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis. Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans. The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products. The selectivity and sensitivity (0.5 fmol μL−1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2. A pipette-tip application for rapid glycoproteomic and glycomic analysis was developed by packing titania-based material into the pipette tips. The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania … |
| Anahtar Kelimeler |
| Enrichment | Glycan | Glycomics | Glycopeptide | Glycoproteomics | Mass | Purification | Spectrometry |
Science Direct
A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 14 |
| Scopus | 18 |
| Google Scholar | 21 |