Fast and efficient proteolysis by reusable pepsin-encapsulated magnetic sol-gel material for mass spectrometry-based proteomics applications
Yazarlar (2)
Doç. Dr. Hacı Mehmet KAYILI Hacettepe Üniversitesi, Türkiye
Prof. Dr. Bekir Salih Hacettepe Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Talanta (Q1) | ||
| Dergi ISSN | 0039-9140 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2016 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-08-2016 |
| Cilt / Sayı / Sayfa | 155 / 1 / 78–86 | DOI | 10.1016/j.talanta.2016.04.014 |
| Makale Linki | http://dx.doi.org/10.1016/j.talanta.2016.04.014 | ||
| UAK Araştırma Alanları |
Analitik Kimya
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| Özet |
| Hydrophobic silicon-based material having magnetic properties was fairly synthesized by a classical sol-gel approach. Pepsin enzyme was encapsulated in the sol-gel material and the enzyme activity was evaluated in consequence of the digestion of some common proteins such as α- and β-casein, cytochrome c, myoglobin, and bovine serum albumin (BSA) both in a single protein batch and in the protein mixture. The optimum digestion time of the studied proteins using pepsin-encapsulated magnetic sol-gel material was found to be 20 min. To produce the magnetic sol-gel material for convenient and easy proteomics applications, Fe3O4 was doped inside sol-gel material during the gelation step. It was observed that the activity of encapsulated pepsin was not affected by the amount of Fe3O4. Poly(ethylene glycol) was also inserted in sol-gel bulk to obtain suitable roughness and increase the hydrophilicity of the … |
| Anahtar Kelimeler |
| Encapsulation | Mass spectrometry | Pepsin | Proteolysis | Proteomics | Sol-gel |
Science Direct
Fast and efficient proteolysis by reusable pepsin-encapsulated magnetic sol-gel material for mass spectrometry-based proteomics applications
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 10 |
| Scopus | 10 |
| Google Scholar | 15 |